Diagnostic Service - Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
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LC-MS/MS combines the powerful analytical separation capability of high performance liquid chromatography (HPLC) with mass spectrometry (MS), an extremely sensitive mass detection technique. LC-MS/MS technology enables ‘high-sensitivity detection’ of conventional as well as specialized clinical chemistry analytes in areas of therapeutic drug monitoring, detection of inborn errors of metabolism (IEM), estimation of 25-hydroxy Vitamin D, measurement of steroids, hormones and many more.


  • We conduct Newborn Screening (NBS) by the most sensitive and accurate method for NBS, LC-MS/MS, to detect more than 40 inborn metabolic disorders. NBS should be performed for babies max. upto 30 days of age (ideally within 3rd-5th days after birth).
  • LC-MS/MS is used for 25 Hydroxy Vitamin D (25OHD) analysis. This method is considered to be the Gold Standard and twofold more sensitive than conventional immunoassays for Vitamin D analysis. It can accurately quantitate both 25OHD forms i.e. 25OHD2 & 25OHD3 which are essential for diagnosis and monitoring of patients with vitamin D disorders.